SUMMARY: Chlamydia trachomatis is one of the most prevalent bacteria found in patients with sexually transmitted infections (STIs), who are almost asymptomatic in society. Two hundred and sixty urine samples of women in two groups (symptomatic and asymptomatic) were collected from patients attending the STI clinic at Mehrad Hospital in Tehran and tested by polymerase chain reaction (PCR) for the presence of C DNA. trachomatis. A total of 39 women in both groups were infected (14.99%), of which 27/130 people were in the symptomatic group (20.76%), compared to 12/130 people in the asymptomatic group (9.23%). ). A significant association was observed between C. trachomatis infections with history of sexually transmitted diseases in both groups dependently and with variable urinary parameters such as white blood cells in each group independently and with epithelial cells only in symptomatic women. The present study shows that screening strategies for chlamydial infections as part of routine investigations, especially for symptomatic married women, are INTRODUCTION Sexually transmitted infections (STIs) are the second leading cause of unpleasant illnesses in young adult women around the world (1). Chlamydia trachomatis genital infection is one of the most common sexually transmitted diseases and is considered the most common treatable and preventable sexually transmitted disease and cause of infertility in women (2). Although C. trachomatis infection has been suggested to be a cause of infertility due to consequences on the genital tract, routine screening for C. trachomatis infections is not performed in many hospitals and clinics. Nowadays, the detection of C. trachomatis infections has improved with the development of nucleic acid-based amplification methods such as polymerase chain reaction (PCR) in urine samples, and most countries have established that To optimize this strategy, sexual health services should become widely available and services should be advertised in a comfortable and attractive manner in Iran. However, asymptomatic people infected with C. trachomatis play a key role in the distribution of the infection and may remain in the shadows and thus continue to spread the infection to partners. Interventions of variable content can lead to promoted changes in information, attitudes, awareness, self-efficacy, skills and behaviors such as the use of appropriate procedures for contraceptive methods, causes of sexual infections and practice of safe sexual ways. Finally, further studies to determine the correlations between infected people and other necessary variables in other cities of Iran. ACKNOWLEDGMENTS This project was supported by Tehran University of Medical Sciences.